![]() Following multiple rounds of optimisations, trials-and-errors and long working hours, I have become a confident user of this technology. It is now April 2016, and I have come a long way. I had never used CRISPR/Cas9 and did not have a clue what conditions would work best in my hands! I would generate these point mutations using CRISPR/Cas9, a technology providing scientists with state-of-the-art molecular DNA scissors. My project involved modelling disease-specific point mutations in human pluripotent stem cells. When I started my PhD project in October 2015, I knew three things for sure: Want to be Benchling’s guest blogger? Contact us. Check out this post for her tips and tricks! Ultimately, she was able to create point mutation knockins in HEK293s using CRISPR with up to 18% efficiency without selection. ![]() Below she shares her journey in harnessing the power of CRISPR. Editor’s note: This post was contributed by Ralitsa Madsen, a PhD student at the University of Cambridge. ![]()
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May 2023
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